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斯氏按蚊性别决定基因fruitless的鉴定及其性别可变剪接分析 认领

Isolation and expression profiling of fruitless gene in Anopheles stephensi
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摘要 目的 分离、鉴定和分析斯氏按蚊性别决定基因fruitless(Anstfru).方法 通过生物信息学方法与分子生物学方法获得了Anstfru基因的全长.通过RT-PCR方法验证Anstfru的性别可变剪接模式与时间表达特点.通过与已知物种FRU蛋白比较,分析斯氏按蚊FRU蛋白结构特点与分子进化特征.结果 分离并鉴定Anstfru基因全长,其在雌、雄蚊中通过可变剪切形成性别特异性mRNA.Anstfru在1~2龄阶段幼虫阶段开始表达,表达量随后逐渐升高,在成蚊中表达量最高.FRU蛋白具有保守的BTB与锌指功能结构域区.结论 Anstfru具有保守的昆虫性别决定基因fru表达特征与功能结构域,对其深入研究将为其最终应用于蚊虫雌雄分离技术,推动昆虫不育技术(Sterile insect technique,SIT)在蚊媒传染病防治上的应用. Objective To isolate,identify and analyze the sex-determining gene fruitless(Anstfru) of malaria-transmitting mosquito Anopheles stephensi.Methods The full length cDNA of the gene Anstfru was obtained by using bioinformatic and molecular biological method.RT-PCRmethod was used to validate the sex variable splicing pattern and expression time characteristics.The structural features and molecular evolutional features of FRU protein of Anopheles stephensi were analyzed via comparison with FRU protein of known species.Results The full length of Anstfru gene was isolated and identified,and sex-specific mRNA of the gene could form in female and male mosquitos through variable splicing.The Anstfru began to be expressed from early 1^st-2^nd stage larvae embryo,the quantity of expression increased subsequently and displayed the highest expression level in adult stage.The FRU protein had the sequence-conservative BTB and zinc finger functional domains.Conclusions Anstfru gene showed conservative functional domains and sex-determining gene fru expression features in mosquito and further in-depth studies on which will facilitate the application of techniques separating female mosquitos from male mosquitoes,and sterile insect technique (SIT)/technology in prevention and treatment of mosquito-borne infectious diseases.
作者 王衍海 王欣玲 顾金保 Wang Yanhai, Wang Xinling, Gu Jinbao(1 State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China ; 2Key Laboratory of Prevention and Control for Emerging Infectious Diseases of Guangdong Higher Institutes, Department of Pathogen Biology, School of Public Health, Southern Medical University, Guangzhou 510515, China )
出处 《中华实验和临床病毒学杂志》 CAS CSCD 2017年第3期245-248,共4页 Chinese Journal of Experimental and Clinical Virology
基金 国家自然科学基金(81672054)
关键词 斯氏按蚊 FRUITLESS 性别决定基因 性别可变剪接分析 Anopheles stephensi Fruitless Sex-determining gene Sex-specific alternative splicing
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