期刊文献+

小干扰RNA抑制RUNX2对人乳腺癌MCF-7细胞增殖、凋亡与侵袭的影响 认领

Inhibition of human RUNX2 by small interfering RNA on proliferation,apoptosis and invasion of human breast cancer MCF-7 cells
收藏 分享 导出
摘要 目的探讨小干扰RNA抑制人RUNT相关转录因子2(RUNX2)对人乳腺癌MCF-7细胞增殖、凋亡与侵袭的影响。方法应用化学合成小干扰RNA技术沉默人乳腺癌MCF-7细胞中RUNX2基因的表达,用逆转录-聚合酶链反应(RT-PCR)和Western blot实验验证基因沉默效率。应用MTT方法检测细胞增殖能力变化,流式细胞仪检测细胞凋亡的变化,Transwell实验检测细胞侵袭能力变化。结果设计的siRNA能够明显抑制人乳腺癌MCF-7细胞RUNX2的表达。RUNX2基因沉默后,人乳腺癌MCF-7细胞的增殖能力降低,细胞凋亡率明显提高,侵袭能力显著降低(P<0.01)。结论RUNX2基因是治疗人乳腺癌MCF-7细胞的潜在靶点。 Objective To investigate the effect of small interfering RNA(SIRNA)inhibiting human RUNTrelated transcription factor 2(RUNX2)on proliferation,apoptosis and invasion of human breast cancer MCF-7 cells.Methods The expression of RUNX2 gene in human breast cancer MCF-7 cells was silenced by chemical synthesis of small interfering RNA,the efficiency of gene silencing was verified by RT-PCR and Western blot.MTT assay was used to detect the change of cell proliferation,flow cytometry was used to detect the change of cell apoptosis,and Transwell assay was used to detect the ability of cell invasion.Results The designed siRNA significantly inhibited the expression of RUNX2 in human breast cancer MCF-7 cells.After RUNX2 gene silencing,the proliferation ability of human breast cancer MCF-7 cells was decreased,the apoptotic rate was increased and the invasive ability was decreased significantly(P<0.01).Conclusion RUNX2 gene is a potential target for the treatment of human breast cancer MCF-7 cells.
作者 刘壮凯 张瑞山 张鑫丰 李翔 白静慧 徐宏 LIU Zhuang-kai;ZHANG Rui-shan;ZHANG Xin-feng;LI Xiang;BAI Jing-hui;XU Hong(Department of Breast Cancer,Cancer Hospital of China Medical University,Liaoning Cancer Hospital&Institute,Shenyang 110042,China)
出处 《解剖科学进展》 2020年第1期51-53,共3页 Progress of Anatomical Sciences
基金 辽宁省科技攻关项目(2013225021)。
关键词 乳腺癌 MCF-7细胞 RUNT相关转录因子2 增殖 凋亡 侵袭 breast cancer MCF-7 cells RUNT-related transcription factor 2 proliferation apoptosis invasion
  • 相关文献

参考文献5

二级参考文献43

  • 1Huang R L, Yuan Y, Tu J, et al. Opposing TNF-alpha/IL-lbeta-and BMP-2-activated MAPK signaling pathways converge on Runx2 to regulate BMP-2-induced osteoblastic differentiation [ J/OL ]. Cell Death Dis, 2014, 5 : e1187, doi: 10. 1038/cddis. 2014. 101. 被引量:1
  • 2~hana A, Callon K E, Pool B, et al. ~tmln'ate n~hydrate crystals inhibit ostooblast viability and function: implications for development of bone erosion in gout [ J]. Ann Rheum Dis, 2011, 70 (9): 1684 - 1691. 被引量:1
  • 3Chen C G, Thuillier D, Chin E N, et al. Chondrocyte-intrinsic Smad3 represses Runx2-inducible matrix metaUoproteinase 13 expression to maintain articular cartilage and prevent osteoarthfitis [J]. Arthritis Rheum, 2012, 64(10): 3278-3289. 被引量:1
  • 4Fu J, Wang S, Lu H, et al. In vitro inhibitory effects of terpenoids from chloranthus multistachys on epithelial-mesenchymal transition via down-regulation of Runx2 activation in human breast cancer [ J]. Phytomedicine, 2015, 22( 1 ) : 165 - 172. 被引量:1
  • 5Li X Q, Du X, Li D M, et al. 1TGBL1 is a Runx2 transcriptional target and promotes breast cancer bone metastasis by activating the TGFI3 signaling pathway [ J ]. Cancer Res, 2015, 75 ( 16 ) : 3302 -3313. 被引量:1
  • 6Choe M, Bmsgard J L, Chumsri S, et al. The RUNX2 transcription factor negatively regulates SIRT6 expression to alter glucose metabolism in breast cancer cells [ J ]. J Cell Biochem, 2015, 116(10) : 2210 -2226. 被引量:1
  • 7Xie J, Yu F, Li D, et al. microRNA-218 regulates cisplatin (DPP) chemosensitivity in non-small cell lung cancer by targeting RUNX2 [J]. Tumour Biol, 2016, 37( 1 ) : 1197 - 1204. 被引量:1
  • 8Yang S, Quaresma A J, Nickerson J A, et al. Subnuclear domain proteins in cancer cells support the functions of RUNX2 in the DNA damage response [J]. J Cell Sci, 2015, 128(4) : 728 -740. 被引量:1
  • 9Yu W, Qiao Y, Tang X, et al. Tumor suppressor long non-coding RNA, MT1DP is negatively regulated by YAP and Runx2 to inhibit FoxA1 in liver cancer cells [J]. Cell Signal, 2014, 26 (12) : 2961 - 2968. 被引量:1
  • 10Zhang X, Wu H, Dobson J R, et al. Expression of the IL-11 gene in metastatic cells is supported by Runx2-Smad and Runx2-c-Jun complexes induced by TGF-β1 [J]. Cell Biochem, 2015, 116(9) : 2098 - 2108. 被引量:1

共引文献7

维普数据出版直通车
今日学术
投稿分析
职称考试

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈